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FUJIFILM multigauge software analysis tool
AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the <t>MultiGauge</t> analysis software.
Multigauge Software Analysis Tool, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multigauge+software+analysis+tool/multigauge+software+analysis+tool/pmc05339766-683-9-7
Average 90 stars, based on 1 article reviews
multigauge software analysis tool - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "DENEDDYLASE1 Protein Counters Automodification of Neddylating Enzymes to Maintain NEDD8 Protein Homeostasis in Arabidopsis * "

Article Title: DENEDDYLASE1 Protein Counters Automodification of Neddylating Enzymes to Maintain NEDD8 Protein Homeostasis in Arabidopsis *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M116.767103

AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the MultiGauge analysis software.
Figure Legend Snippet: AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the MultiGauge analysis software.

Techniques Used: SDS Page, Labeling, Western Blot, Mutagenesis, Generated, Software

Related Articles

Western Blot:

Article Title: DENEDDYLASE1 Protein Counters Automodification of Neddylating Enzymes to Maintain NEDD8 Protein Homeostasis in Arabidopsis
Article Snippet: .. Immunoblot signal intensities were quantified with the Fuji Film MultiGauge software analysis tool. .. Chemical Treatments For NEDD8 inhibition, seedlings were grown for 7 days under continuous light on growth medium supplemented with 10 μ m MLN4924 (Millennium Pharmaceuticals, Cambridge, MA).

Software:

Article Title: DENEDDYLASE1 Protein Counters Automodification of Neddylating Enzymes to Maintain NEDD8 Protein Homeostasis in Arabidopsis
Article Snippet: .. Immunoblot signal intensities were quantified with the Fuji Film MultiGauge software analysis tool. .. Chemical Treatments For NEDD8 inhibition, seedlings were grown for 7 days under continuous light on growth medium supplemented with 10 μ m MLN4924 (Millennium Pharmaceuticals, Cambridge, MA).



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90
FUJIFILM multigauge software analysis tool
AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the <t>MultiGauge</t> analysis software.
Multigauge Software Analysis Tool, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multigauge+software+analysis+tool/multigauge+software+analysis+tool/pmc05339766-683-9-7
Average 90 stars, based on 1 article reviews
multigauge software analysis tool - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
FUJIFILM fuji film multigauge software analysis tool
AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the <t>MultiGauge</t> analysis software.
Fuji Film Multigauge Software Analysis Tool, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multigauge+software+analysis+tool/fujifilm+multigauge+software/10__1074_slash_jbc__m116__767103-200-9-7
Average 90 stars, based on 1 article reviews
fuji film multigauge software analysis tool - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the MultiGauge analysis software.

Journal: The Journal of Biological Chemistry

Article Title: DENEDDYLASE1 Protein Counters Automodification of Neddylating Enzymes to Maintain NEDD8 Protein Homeostasis in Arabidopsis *

doi: 10.1074/jbc.M116.767103

Figure Lengend Snippet: AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the MultiGauge analysis software.

Article Snippet: Immunoblot signal intensities were quantified with the Fuji Film MultiGauge software analysis tool.

Techniques: SDS Page, Labeling, Western Blot, Mutagenesis, Generated, Software